|
HS Code |
223943 |
| Product Name | Phenol Red Free Acid Cell Culture Tested |
| Form | Powder |
| Molecular Formula | C19H14O5S |
| Molecular Weight | 354.38 g/mol |
| Appearance | Red to dark red powder |
| Solubility | Water soluble |
| Grade | Cell culture tested |
| Storage Temperature | 2-8°C |
| Cas Number | 143-74-8 |
| Use | pH indicator in cell culture |
| Ph Range | 6.8 (yellow) to 8.2 (red) |
| Synonyms | Phenolsulfonphthalein free acid |
| Light Sensitivity | Light sensitive |
| Absorption Maximum | 558 nm |
| Expiration | Refer to product label |
As an accredited Phenol Red Free Acid Cell Culture Tested factory, we enforce strict quality protocols—every batch undergoes rigorous testing to ensure consistent efficacy and safety standards.
| Packing | The packaging is a 25g amber glass bottle, labeled "Phenol Red Free Acid Cell Culture Tested," ensuring protection from light and contamination. |
| Shipping | Phenol Red Free Acid Cell Culture Tested is shipped at ambient temperature in tightly sealed containers to protect from moisture and contamination. The package is clearly labeled as a laboratory chemical. Fast, reliable delivery ensures the product retains its quality and is suitable for immediate use in research or cell culture applications. |
| Storage | Phenol Red Free Acid, Cell Culture Tested, should be stored tightly sealed at 2–8°C in a dry, well-ventilated area, away from light and incompatible substances. Always avoid moisture and contamination by keeping the container tightly closed when not in use. Proper storage ensures stability and maintains suitability for sensitive cell culture applications, preventing interference in assays that require phenol red-free conditions. |
Competitive Phenol Red Free Acid Cell Culture Tested prices that fit your budget—flexible terms and customized quotes for every order.
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The selection of every component in a cell culture medium directly shapes the health and reproducibility of any experiment. Experienced cell biologists know that phenol red, often added as a pH indicator in basal media, can become more than a simple colorimetric tool. Its chemical structure interacts with cellular activities, particularly in more sensitive assays or cell types. We manufacture Phenol Red Free Acid, cell culture tested, specifically for research settings that demand absolute minimal interference from background molecules.
Our facility produces Phenol Red Free Acid with careful control over each synthesis and purification step. We focus on keeping trace contaminants below detection limits for use in exacting laboratory environments. Researchers working with hormone-sensitive cells or those relying on high-throughput imaging benefit from an indicator-free medium, avoiding background fluorescence or mimicry of estrogenic activity. Unlike general laboratory chemicals, our Phenol Red Free Acid is tailored through each batch for cell culture, with strict microbial, heavy metal, and purity testing.
Many modern cell-based experiments—especially those monitoring hormone pathways or using fluorescence-based assays—suffer from subtle biases when phenol red is present. Common cell lines like MCF-7 and LNCaP, widely used in endocrine research, show altered gene expression and proliferation patterns even at low indicator concentrations. Avoiding phenol red greatly improves the accuracy of these readouts, reducing false positives and supporting regulatory submission of pharmaceutical and biotechnological data.
We supply Phenol Red Free Acid as a high-purity crystalline powder, formula C19H14O5S, with strict batch-to-batch consistency. Our cell culture tested grade has the following physical properties:
We exclude any carriers or preservatives. After synthesis, each lot is tested against common contaminants that can trigger cell stress, with independent confirmation on both fibroblast and epithelial cell lines. Bioburden testing includes both aerobic and anaerobic bacteria, as well as fungal exclusion.
Phenol red-free formulations offer a clear advantage in live-cell imaging—no background tint, no spectral overlap, and less autofluorescence. In our own internal studies, media without phenol red allows detection of low-abundance signals at earlier time points, and cell morphologies remain easier to discern under phase contrast objectives.
Researchers in neurobiology and stem cell fields have struggled with unexpected results during time-lapse microscopy—simply removing the phenol red resolves many of these misreadings. This is not just a theoretical concern. Actual results from imaging human iPSC-derived neurons showed sharper contrast and more reliable quantification with phenol red-free media, saving on time and consumables.
Phenol red, although commonly thought of as inert, can activate estrogen receptors at concentrations used in tissue culture. Endocrine labs often discard weeks or months of work due to unforeseen agonist activity from their buffer systems. A spike in cell proliferation or unexpected receptor translocation usually traces back to this colored indicator. Our Phenol Red Free Acid leaves these potential variables outside your experiment.
The risk becomes even greater in 3D cultures. Hydrogels and bioprinted scaffolds amplify subtle hormonal cues, making indicator-free media almost essential in organoid research and tissue engineering. This is not a minor adjustment—it's a safeguard to help data withstand peer review and industrial scrutiny.
Pharmaceutical and cancer research regularly calls for unambiguous assay conditions. We make our cell culture tested Phenol Red Free Acid for use in drug discovery pipelines, where compound screening lives or dies by clean controls. Many anti-cancer drug candidates interfere with colorimetric readings, or bind weakly to phenols, throwing off absorbance and fluorescence measurements. With phenol red absent, results speak solely to the cellular response—not to artifacts from the medium.
Cell-based toxicity testing for food and environmental safety also benefits from the removal of this indicator dye. Reporter gene assays and high-content screening platforms show fewer false positives and improved Z' factors in the absence of phenol red—outcomes we have confirmed during beta trials with industrial clients. Scientists in these fields get the latitude to analyze authentic cell behavior.
Regular phenol red powder sold for titration, pH detection, or basic lab use follows very different specifications and purity benchmarks. Many of these products contain residual solvents or buffer salts, which prove unsuitable for mammalian cell maintenance. Furthermore, pre-blended cell culture media marketed as “phenol red-free” sometimes still show traces of indicator due to shared production equipment or ante-room contamination.
Our Phenol Red Free Acid undergoes dedicated vessel cleaning and air handling, a minimum for manufacturing to support GLP and GMP-compliant research. Each production run runs through negative control cell assays, not just chemical analytics. Our documentation reflects decades of feedback from the cell culture community, with continuous improvements driven by findings at the bench, not marketing claims.
Lab technicians and principal investigators alike deal with day-to-day realities such as repeated bottle openings, variable bench conditions, and shared workspace equipment. Our Phenol Red Free Acid keeps well when sealed in a dry, cool environment, away from direct sunlight. Hygroscopicity remains a non-issue under normal storage; compaction or agglomeration rarely happens, avoiding delays during weighing or medium prep.
The lack of strong odors and non-hazardous dusting makes it safer to handle during bench-scale and pilot production. Even after weeks in use, the product holds consistency for both hand-dispensed and automated buffer prep. Our on-site QC team reviews each lot for changes in color or solubility before it leaves the warehouse.
Based on direct customer feedback and our own use cases, the most frequent applications include:
We also see significant uptake for custom 3D matrix work, where any microenvironmental variable becomes amplified over time. Laboratories focusing on patient-derived organoids in cancer research, developmental biology, or tissue modeling repeatedly cite the lack of phenol red as reducing batch-to-batch signal noise.
Institutes preparing for FDA or EMA approval require every ingredient to hold up under audit. Our manufacturing logs detail every critical variable—starting materials, temperature, time, and handling steps—mapped directly to each batch code. Each bottle ships with a signed Certificate of Analysis including heavy metal, microbial, and endotoxin results. Test results come from real cell line challenge, not just thin-layer or chromatography readings. Every deviation, even minor, triggers an immediate product hold pending investigation, ensuring you receive nothing but fully compliant product.
We keep all test records and production data available years after delivery, in line with regulatory guidelines for pharmaceutical submissions. You gain both transparency and security, with no last-minute surprises during preclinical or clinical documentation.
The “cell culture tested” designation comes from direct, repeated exposure to living cells over defined time frames, under both stress and baseline conditions. Our product demonstrates the ability to support healthy cell growth, with no detectable cytotoxicity or morphological changes at normal-use dilutions. Beyond sterility, we check for leachates, particulates, and activation of common cellular pathways that can complicate sensitive studies.
We regularly review literature and coordinate with outside cell culture facilities to update our acceptance criteria. Customer reports feed back into our process—if an unexpected issue arises, we trace the source and update future batches. This active process of iteration and feedback distinguishes our product’s reliability and continues to drive improvements.
Transitioning from traditional basal media to a phenol red-free environment does not create workflow headaches when handled with planning. Researchers often preload their protocols by comparing cultures in matched control and phenol red-free conditions, establishing baseline differences in gene expression, morphology, and viability before introducing experimental variables.
Based on user reports and our own internal observations, most common extracellular pH shifts are easily compensated for by adopting pH meters or non-interfering color indicators as needed. Visual tracking in daily maintenance takes little adjustment for experienced teams, while novice technicians quickly grow accustomed to the clear media. Our support specialists frequently field queries about switching, and can provide tips for calibration and documentation during protocol changes.
As the demand for reproducible, clean cell culture systems grows, sustainable production takes a larger role in chemical manufacturing. In our plant, waste reduction and environmentally sound disposal follow every production batch of Phenol Red Free Acid. We maintain containment procedures for spent solvents and minimize resource use by optimizing crystallization and drying cycles.
Lab-scale biologists see indirect benefit as well—less contamination risk and less product needed to achieve the same results. Reduced false positives and repeat assays lower not just material cost but energy and labor inputs. This holistic chain of reliability helps keep facility sustainability targets in sight. We continue adapting equipment and workflow around improvements in chemical process design, specifically for cell culture reagents.
No process runs perfectly in perpetuity, and continual feedback from cell culture teams drives how we produce and test phenol red-free products. Issues with early batches—traces of sodium left from neutralization, or occasional batch-to-batch solubility variances—taught us to invest in additional rinses and ultrapure water cycles. Improvements in powder handling equipment prevented micro-contamination, a seemingly minor update that saved countless hours of troubleshooting downstream.
We keep our line open to real needs coming from research fields as diverse as oncology, synthetic biology, regenerative medicine, and food tech startups. Every bottle of cell culture tested Phenol Red Free Acid reflects both chemical rigor and bench experience. Future upgrades will likely address more defined particle sizing and faster solubilization tailored to automation-friendly liquid handling robots.
We also collaborate closely with academic facilities and industry partners to run blinded testing of each lot against evolving cell models. The goal continues to center on removing uncertainty in every stage of the cell culture workflow, from upstream thawing and passaging to downstream analysis and archiving.
We do not outsource our manufacturing or quality control work. Every process step carries strict oversight—enzyme deactivation, vacuum drying, and sterile filling—run by seasoned staff with years handling sensitive research chemicals. Each improvement gets evaluated in-house for impact on purity, safety, and batch reliability; shortcuts never enter our workflow.
Our ability to adapt output scale without losing lot-to-lot reproducibility comes from technical skill on the shop floor and real collaboration with our customers. Regulatory and research trends change rapidly, but our consistency enables broad adoption among both established pharmaceutical labs and high-growth startups. Time and again, we hear back from groups that our product solves stubborn background artifacts or improves high-content screening statistics thanks to its tested purity and handling traits.
Ultimately, our Phenol Red Free Acid, cell culture tested, allows ambitious research programs to trust every detail of their experimental setup—leaving only the true biology in the spotlight.
